P5 Analisis Lemak atau Minyak Biokimia

Assalamualaikum warahmatullahi wabarakatuh Hi friends of the chemistry this time we will carry out a biochemistry practice with the title of quality analysis of fat or oil even though the purpose of this experiment is

Students can learn one of the testing methods for chemical ingredients, namely testing for fat or oil. Fat or oil is triglycerides or triacylglycerol and is often also called triesterglycerol.

Fat or oil can be damaged due to the oxidation process of oxygen from the air. There are also parameters that determine the quality of fat or oil including the number of peroxides, the number of solubilizers, the number of iodine,

which is an important parameter to determine the profile of certain fatty acids based on the combination of fiber and ester. The amount of peroxides is many milligrams of the peroxide equivalent that is formed in every 1000 grams of fat or oil.

while the amount of the mixture is the amount of KOH needed to mix 1 gram of oil or fat. The more KOH needed to mix the amount of fatty acids in fat or oil, the higher the amount of fatty acids in the sample.

There is also a method used to determine the quality parameters of fat or oil, which is the titration method.

There are also work procedures in this experiment, including the first step is to determine the number of peroxides

For the measurement of the number of peroxides, a titration volume measurement is done for the solution of the blank and sample. The first step is to measure as much as 1 gram of aquadesc as a blank and 1 gram of oil sample each is put into Erlenmeyer 250 ml.

Then, peroxide solution is made by mixing chloroform and acetic acid with a ratio of 2:3 volume per volume

then from the mixture, take as much as 30 ml and put it in the 250 ml Erlenmeyer which contains blanko and sample

Next, add 1 ml of potassium iodide solution and dilute the sample with 30 ml of aquadesc and do the mixing until homogen

Next, a sample is titrated with a standard solution of 0.05 molar sodium thiosulfate until color changes. This titration process is done in duplo.

Then, the same step is done on the oil sample. The second step is to determine the number of the solution.

For the measurement of the concentration, the first is to do a volumetric measurement for the Blanco solution. Take 25 ml of 0.5 MHg KH solution in ethanol and put it in Erlenmeyer 250 ml.

Then add 3 drops of phenolphthalein indicator and titration using the standard solution of HCl 0.5 molar until color change occurs. This titration process is done in duplex.

Next, determine the volume of the sample titration. measure 4 grams of fat or oil sample then put it in the 250 ml Erlenmeyer

Then add 25 ml of 0.5 molar hydroxide helium solution in ethanol with a ratio of 1:2. Next, heat it until soap formation occurs.

After heating, then cool until the soap is formed. Next, add 3 drops of phenolphthalein indicator and titrate with a solution of HCl 0.5 molar until color change occurs. This titration process is done in duplo.

then, calculate the titration volume and calculate the total number of the addition